Table 1. Histological placements of experimental animals.

Table shows anatomical confirmed placement ranges for experimental animals targeting either lateral central amygdala (CeA), nucleus accumbens (NAc) shell, or dorsolateral bed nucleus of stria terminalis (BNST). Confirmed placement ranges are determined from Paxinos and Watson brain atlas (50) and display anterior/posterior (A/P), medial/lateral (M/L), and dorsal/ventral (D/V) coordinates in mm from bregma. N-values for excitatory channelrhodopsin (ChR2), inactive control eYFP virus, and inhibitory halorhodopsin (NpHR) groups include those with bilateral fiber and virus placements (Bil) or unilateral virus/fiber placements in one hemisphere, with a contralateral miss in the other hemisphere (Uni). For CeA rats (top), contralateral miss sites were located in either basolateral amygdala (BLA), medial amygdala (MeA), or optic tract, with no substantial virus expression found in these missed hemispheres. For NAc rats (middle), placements for unilateral misses were located in dorsal striatum (DS), medial septum (MS), or nucleus accumbens core (NAcC) and no substantial viral expression in these structures was observed. For BNST rats (below) sites of unilateral misses in either the anterior commissure (ac) or globus pallidus (GP) without substantial virus expression. See Fig. 1.

Target	Confirmed placement ranges (mm from Bregma)			ChR2 N’s		eYFP N’s		NpHR N’s		Contralateral misses, locations
	A/P	M/L	D/V	Uni	Bil	Uni	Bil	Uni	Bil
CeA	−2.16 to −3.00	± 4.2 to 4.7	−7.0 to −7.6	3	7	2	5	3	4	BLA, MeA, optic tract
NAc	+1.44 to 0.96	± 0.8 to 1.6	−6.3 to −7.6	7	5	3	3	3	3	DS, MS, NAcC
BNST	+0.24 to −0.24	±1.6 to 2.0	−5.8 to −6.4	4	7	3	3	2	4	ac, GP