| Add additional rows as needed for each resource type |
Include species and sex when applicable. |
Include name of manufacturer, company, repository, individual, or research lab. Include PMID or DOI for references; use “this paper” if new. |
Include catalog numbers, stock numbers, database IDs or accession numbers, and/or RRIDs. RRIDs are highly encouraged; search for RRIDs at https://scicrunch.org/resources. |
Include any additional information or notes if necessary. |
| Antibody |
rabbit anti-cFos |
Synaptic Systems, Göttingen, Germany |
Catalog#: 226 003; Lot #: 4–63; RRID:AB_2231974 |
1:2500 dilution |
| Antibody |
chicken anti-GFP |
Abcam, Cambridge, MA |
Catalog#: AB13970; Lot #: GR3190550–30; RRID:AB_300798 |
1:2000 dilution |
| Antibody |
Biotin-SP donkey anti-rabbit |
Jackson Immunoresearch, West Grove, PA |
Catalog #: AB2340593; Lot #: 128703; RRID:AB_2340593 |
1:300 dilution |
| Antibody |
donkey anti-chicken AlexaFluor 488 |
Jackson Immunoresearch, West Grove, PA |
Code #: AB2340375; Lot #: 144438; RRID:AB_2340375 |
1:300 dilution |
| Antibody |
Streptavidin Cy3 |
Jackson Immunoresearch, West Grove, PA |
Catalog #: AB2337244, Lot #: 141873, RRID:AB_2337244 |
1:300 dilution |
| Bacterial or Viral Strain |
AAV-EF1a-DIO-hChR2(H134R)-EYFP |
University of North Carolina Vector Core |
N/A |
|
| Bacterial or Viral Strain |
AAV-EF1a-DIO-EYFP |
University of North Carolina Vector Core |
N/A |
|
| Bacterial or Viral Strain |
AAV-EF1a-DIO-eNpHR3.0-EYFP |
University of North Carolina Vector Core |
N/A |
|
| Chemical Compound or Drug |
Atropine |
Henry Schein |
N/A |
0.05mg/kg; i.p. |
| Chemical Compound or Drug |
Cefazolin |
Henry Schein |
N/A |
75mg/kg, s.c. |
| Chemical Compound or Drug |
Carprofen |
Henry Schein |
N/A |
5mg/kg, s.c. |
| Commercial Assay Or Kit |
RNAscope® Multiplex Fluorescent Reagent Kit v2 |
Advanced Cellular Diagnostics |
Cat. No. 323100 |
|
| Organism/Strain |
Rat: Crh-Cre, male and female |
Messing Lab, University of Texas |
RRRC#: 00852 |
|
| Sequence-Based Reagent |
Rn-Crem-03 probe |
Advanced Cellular Diagnostics |
Cat. No. 530001 |
|
| Sequence-Based Reagent |
Rn-Crh-C3 |
Advanced Cellular Diagnostics |
Cat. No. 318931-C3 |
|
| Software; Algorithm |
Matlab |
Mathworks |
RRID:SCR_001622 |
|
| Software; Algorithm |
Surveyor with Turboscan |
Objective Imaging LTD. |
RRID:SCR_014433 |
|
| Software; Algorithm |
MED-PC IV |
MedAssociates |
RRID:SCR_012156 |
|
| Software; Algorithm |
Adobe Illustrator 23.0 |
Adobe Inc. |
RRID:SCR_010279 |
|
| Software; Algorithm |
Observer XT 12 |
Noldus |
RRID:SCR_004074 |
|
