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. Author manuscript; available in PMC: 2022 Jun 3.
Published in final edited form as: Structure. 2021 Feb 1;29(6):572–586.e6. doi: 10.1016/j.str.2021.01.001

Figure 5. Crosslinking studies show that PACRG:MEIG1 and PACRGL bind to tubulin.

Figure 5.

(A) SDS-PAGE analysis of crosslinking reactions between taxol-polymerized tubulin (5 μM) and different candidate microtubule-associated proteins (12.5 μM, except DCX at 1.5 μM). Gels were imaged by UV-induced fluorescence. Interactions between tubulin and candidate proteins are detected by the loss of tubulin α/β dimers (→) and appearance of tubulin-protein trimers or tetramers (*). (B) Densitometry analysis of crosslinked bands highlighted in A, shown as a ratio of the two bands, following background subtraction (tubulin alone). One-way ANOVA and Dunnett’s tests were performed for comparison with either BSA (gel 1) or wild-type (WT) PACRG:MEIG1 (gels 2–4). * indicates p ≤ 0.05, ** for p ≤ 0.01, *** for p ≤ 0.001, ns, not significant (n=2).