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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Mol Cancer Res. 2021 Mar 11;19(6):1005–1014. doi: 10.1158/1541-7786.MCR-20-0534

Figure 2. Identify potential YAP target genes that regulate ferroptosis through integrated genomics.

Figure 2.

(A) The mRNA expression levels of YAP and WWTR1 (TAZ) were determined by RT-qPCR after siRNA-mediated YAP knockdown in RCC4 cells. n=3; mean ± SEM; one-way ANOVA; n.s.: not significant; ***p < 0.001; ****p < 0.0001.

(B) The heatmap of the transcriptome response of RCC4 cells to YAP knockdown without (-) or with (+) erastin treatment. n=3 per group.

(C) Venn diagram showing genes that were both downregulated upon YAP knockdown and identified as hits in the siRNA ferroptosis screen.

(D) RT-qPCR was used to validate the downregulation of SKP2 mRNA level upon YAP knockdown in RCC4 cells. n=3; mean ± SEM; one-way ANOVA; n.s.: not significant; ****p < 0.0001.

(E) RNA-seq revealed that SKP2 mRNA level was downregulated when YAP was knockdown in CAOV2 cells. n=3; mean ± SEM; Student’s t-test; ****p < 0.0001.

(F) Western blots validated that YAP knockdown decreased SKP2 protein level in MDA-MB-231 cells.

(G) GSEA analyses show the YAP knockdown in RCC4 cells led to the depletion of several cell cycle-related genesets.