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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Cancer Res. 2021 Mar 25;81(11):2956–2969. doi: 10.1158/0008-5472.CAN-20-3450

Fig. 4. NCOA3 targeting in melanoma cells results in mitotic catastrophe.

Fig. 4.

(A) Representative bright field images of C8161.9 and Ma-Mel-12 cells treated with DMSO or bufalin. (B) Western analysis of PLK1, AURKA, CDK1 and GAPDH proteins in Ma-Mel-12 cells stably expressing anti-NCOA3 shRNA or anti-luc shRNA. (C) Western analysis of PLK1, AURKA, CDK1 and GAPDH proteins in C8161.9 and Ma-Mel-12 cells treated with DMSO or bufalin. (D) Representative images of immunofluorescence detecting pericentrin (red) and tubulin (green) in Ma-Mel-12 cells stably expressing anti-NCOA3 shRNA or anti-luc shRNA. (E) Representative images of immunofluorescence detecting pericentrin (red) and tubulin (green) in C8161.9 and Ma-Mel-12 cells treated with DMSO or bufalin. All scale bars are 20 μm.