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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Mol Cancer Res. 2021 Mar 5;19(6):1076–1084. doi: 10.1158/1541-7786.MCR-20-0652

Figure 1. Validation of clonal CRISPR knock-out (KO) cell lines.

Figure 1.

Single cell clone phenotypes were confirmed by flow analysis of H2-Kb, H2-Db and Qa-1b cell surface expression. Two sets of single cell clones were selected and used in mouse experiments. Values are shown as mean ± SEM. (A) Flow cytometry of independent clones after 48 h incubation with IFNγ (100 IU/mL). (B) Representative flow cytometry plots.