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. Author manuscript; available in PMC: 2022 Jun 2.
Published in final edited form as: Neuron. 2021 Apr 15;109(11):1848–1860.e8. doi: 10.1016/j.neuron.2021.03.033

Figure 6. Inhibition of the PMd-cck→dlPAG projection decreases escape in all threat assays while inhibition of the PMd-cck→amv-syn projection selectively decreases context-specific escape.

Figure 6.

(A) Strategy to optogenetically inhibit arch-expressing PMd-cck axons terminating in the dlPAG (scale bar: 100μm)

(B) Optogenetic inhibition of the PMd-cck→dlPAG projection with green light (532 nm) decreased escape in all assays. (rat: YFP n=15, arch n=11; heated floor: YFP n=12, arch n=11; CO2: YFP n=14, arch n=11, Two-way repeated measures ANOVA followed by Wilcoxon test, ***p < 0.001)

(C) Similar to (A), but with fiber optic cannula implanted over the amv. (scale bar: 150μm)

(D) Optogenetic inhibition of the PMd-cck→amv-syn projection with green light decreased escape count during rat (left), heated floor (middle) but not CO2 (right) exposure. (rat: YFP n=9, arch n=12; heated floor: YFP n=9, arch n=12; CO2: YFP n=9, arch n=12, Two-way repeated measures ANOVA followed by Wilcoxon test, ***p < 0.001) Mean ± SEM.