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. 2021 May 5;25(11):5082–5098. doi: 10.1111/jcmm.16491

FIGURE 4.

FIGURE 4

miR‐454‐dependent downregulation of NEDD4‐2 attenuates H9c2 cell apoptosis and injury. H9c2 cells co‐transfected with mimic NC + oe‐NC, mimic NC + oe‐NEDD4‐2, miR‐454 mimic + oe‐NC or miR‐454 mimic + oe‐NEDD4‐2 for 48 h. A, The expression of miR‐454 in H9c2 cells determined by RT‐qPCR; */# P < .05. B, C The mRNA and protein expression of NEDD4‐2 in H9c2 cells determined by RT‐qPCR and Western blot assay; */# P < .05. D, ROS levels in H9c2 cells; */# P < .05. E, The activities of SOD in H9c2 cells as examined by ELISA; */# P < .05. F, The activities of CAT in H9c2 cells as measured by ELISA; */# P < .05. G, The survival rates of H9c2 cells as examined by MTT assay; */# P < .05. H, The apoptotic rates of H9c2 cells examined by flow cytometry; */# P < .05. I, The protein expression of Bax, cleaved caspase‐3 and Bcl‐2 in H9c2 cells determined by Western blot assay; */# P < .05. Measurement data are displayed as mean ± standard deviation. One‐way ANOVA was performed for comparison of data among multiple groups. Each cell experiment was repeated three times