FIGURE 8.

miR‐454 activates cAMP pathway through NEDD4‐2/TrkA axis to delay HF in vivo. The rats were grouped into sham‐operated rats, and HF rats treated with PBS, AAV‐NC, AAV‐miR‐454, and AAV‐miR‐454 + H‐89, respectively. A, The expression of miR‐454 in the heart tissues determined by RT‐qPCR; n = 12; */^#/&P <.05. B, The expression of NEDD4‐2 in the heart tissues determined by RT‐qPCR; n = 12; */^#/&P < .05. C, The protein expression of NEDD4‐2 and TrkA and the extents of PKA and CREB phosphorylation in the heart tissues as measured by Western blot assay; n = 12; */^#/&P < .05. D, The cardiac functions as examined by colour Doppler ultrasound (EF, FS, LVED, LVES and heart rate); n = 12; */^#/&P < .05. E, The degree of myocardial fibrosis as examined by Masson's Trichrome staining; n = 12. F, The apoptotic rate in myocardial tissues as examined by TUNEL staining; n = 12; */^#/&P < .05. G, The protein expression of Bax, cleaved caspase‐3 and Bcl‐2 in heart tissues as examined by Western blot assay; n = 12; */^#/&P < .05. H, The expression of BNP in plasma as examined by ELISA; n = 12; */^# &P < .05. I, The expression of CK‐MB in plasma as examined by ELISA; n = 12; */^# &P < .05. Measurement data are displayed as mean ± standard deviation. One‐way ANOVA was performed for comparison of data among multiple groups