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. 2021 May 5;25(11):5238–5249. doi: 10.1111/jcmm.16531

FIGURE 3.

FIGURE 3

Photobiomodulation therapy (PBMT) could promote ATP‐binding cassette transporters A1 (ABCA1) expression and ABCA1‐dependent cholesterol efflux. A, Western blot (WB) analysis and quantification of ABCA1 expression in primary mouse peritoneal macrophages or lipid‐loaded macrophages after treated with PBMT (n = 6). B, qPCR analysis of ABCA1 expression in primary mouse peritoneal macrophages or lipid‐loaded macrophages after treated with PBMT or positive control T0901317 (n = 8). C, D, WB analysis and quantification of SR‐AI, SR‐BI and CD36 expression in primary mouse peritoneal macrophages or lipid‐loaded macrophages after treated with PBMT (n = 4). E, qPCR analysis of SR‐AI, SR‐BI and CD36 expression in primary mouse peritoneal macrophages or lipid‐loaded macrophages after treated with PBMT (n = 8). F, Representative ABCA1 staining of mouse peritoneal macrophages showing ABCA1 expression after treated with PBMT. Scale bars, 50 μm. G, apolipoprotein A‐I (ApoA‐I) mediated cholesterol efflux after treat with PBMT or DIDS (n = 6). Data are shown as mean ± SEM, control vs PBMT, unpaired two‐tailed Student's t test or one‐way ANOVA. *P < .05, **P < .01, ***P < .001, # P < .05, ## P < .01, ### P < .001, ns = not significant