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. 2021 May 5;25(11):5238–5249. doi: 10.1111/jcmm.16531

FIGURE 5.

FIGURE 5

Activation of phosphatidylinositol 3‐kinases/protein kinase C zeta/specificity protein 1 (PI3K/PKCζ/SP1) signalling cascade by Photobiomodulation therapy (PBMT) promoted ATP‐binding cassette transporters A1 (ABCA1) expression and cholesterol efflux. A‐C, Western blot (WB) analysis of ABCA1 expression in primary mouse peritoneal macrophages with different treatments: Mithramycin A (MTA, 200 nmol/L) A, GÖ6983 (4 μmol/L) B, and wortmannin (WMN, 200 nmol/L) C, (n = 4). D, Representative ABCA1 staining in primary mouse peritoneal macrophages with different treatments. Scale bars, 50 μm. E‐H, Immunoprecipitation (IP) of SP1 and WB E, and statistical analysis of SP1 threonine F, serine G, residues phosphorylation and PKCζ bound to SP1 H, after treated with or without PBMT, WMN, GÖ6983 and MTA (n = 3). I‐K, WB I, and statistical analysis of PKCζ phosphorylation J, and ABCA1 expression K, after treated with PBMT, WMN and GÖ6983 (n = 3). L, Apolipoprotein A‐I (ApoA‐I)‐mediated cholesterol efflux after treat with or without PBMT, WMN, GÖ6983 and MTA (n = 6). Data are shown as mean ± SEM, one‐way ANOVA *P < .05, **P < .01, # P < .05, ## P < .01, ns = not significant