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. 2021 May 3;25(11):4922–4937. doi: 10.1111/jcmm.16280

FIGURE 4.

FIGURE 4

miR‐486‐5p is a downstream miRNA of lnc‐ISG20. A, Targetscan showing the binding of lnc‐ISG2 to miR‐486‐5p. B, The relative expression of miR‐486‐5p in kidney tissues of normal mice (n = 10) and DN mice (n = 10) determined by RT‐qPCR; C, The expression of miR‐486‐5p in MCs under NG and HG treatment conditions determined by RT‐qPCR. D, Verification of the binding of lnc‐ISG20 to miR‐486‐5p by dual‐luciferase reporter assay. E, The localization of lnc‐ISG20 and miR‐486‐5p in MCs identified by FISH. F, Verification of the binding of lnc‐ISG20 and miR‐486‐5p by RIP experiment. G, Under HG treatment condition, the expression of miR‐486‐5p in MCs after lnc‐ISG20 silencing determined by RT‐qPCR. H, Under NG treatment condition, the expression of miR‐486‐5p in MCs after lnc‐ISG20 overexpression determined by RT‐qPCR. *P < .05 compared with Normal mice, NG‐treated MCs, 293T cells treated with NC mimic, MCs treated with IgG antibody, MCs treated with HG + sh‐NC or MCs treated with NG + oe‐NC (The data results are measurement data showed by mean ± standard deviation. Two groups were compared by independent sample t test. The experiment was repeated 3 times)