FIGURE 7.

Lnc‐ISG20 promotes fibrosis of MCs through miR‐486‐5p/NFAT5/p‐AKT. A, The mRNA levels of NFAT5 in MCs after treatment with oe‐lnc‐ISG20/oe‐NC + sh‐NC/sh‐NFAT5 determined by RT‐qPCR. B, The expression of NFAT5, p‐AKT, AKT in MCs after treatment with oe‐lnc‐ISG20/oe‐NC + sh‐NC/sh‐NFAT5 determined by RT‐qPCR. C, The protein expression of collagen IV, fibronectin and TGF‐β1 in MCs under unified HG treatment conditions after treatment with oe‐lnc‐ISG20/oe‐NC + sh‐NC/sh‐NFAT5 measured by Western blot assay. *P < .05 as compared to MCs treated with oe‐NC + sh‐NC. #P < .05 as compared to the MCs treated with oe‐lnc‐ISG20 + sh‐NC. (The data results are measurement data expressed by mean ± standard deviation. One‐way analysis of variance was used for comparisons among multiple groups followed by Tukey's post hoc test. The experiment was repeated 3 times)