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. 2021 May 4;25(11):5326–5334. doi: 10.1111/jcmm.16544

FIGURE 4.

FIGURE 4

miR‐122 directly targets Hand2 to decrease Hand2 expression. (A) Putative binding sites for miR‐122 in the 3’‐UTR of Hand2 identified via bioinformatics program TargetScan. (B) Effect of miR‐122 on the protein expression of Hand2 in mice. Top, examples of Western blot bands; bottom, quantitative analysis. (C) mRNA expression of Hand2 in mice. (D) Expression of miR‐122 in H9C2 cells infected with miR‐NC or miR‐122 mimics. (E) Effect of miR‐122 mimics on the expression of Hand2 in H9C2 cells. Top, examples of Western blot bands; bottom, quantitation analysis. (F) Normalized luciferase ratios in 293T cells that were infected with miR‐NC or miR‐122 mimics, followed by transfection with plasmid constructs containing wild‐type (wt), mutation (mut) of the 3’‐UTR of Hand2. N = 5 samples per group. *P < 0.05, **P < 0.01, ****P < 0.0001 for all panels vs NTG or miR‐NC. An unpaired t test was used to determine statistical significance. Data are means ± SEM