FIGURE 5.

Hand2 silencing increases Drp1 expression and apoptosis demonstrated with H9C2 cells transfected with NC‐OE, Hand2‐OE, si‐NC or si‐Hand2. (A) Verification of transfection effect with Western blot. (B) Representative images of Annexin V‐FITC/PI staining FACS assay for apoptosis. Cells in the lower right quadrant represent early apoptosis and those in the upper right quadrant late apoptosis. (C) Quantitative analysis of apoptosis. (D) Representative expression of Hand2 mRNA in all groups. (E) Effect of Hand2 on the expression of Drp1 in H9C2 cells. Top, examples of Western blot bands; bottom, quantitation analysis. N = 5 samples per group. **P < 0.01, ***P < 0.005 for all panels vs NC‐OE or si‐NC. An unpaired t test was used to determine statistical significance. Data are means ± SEM