FIGURE 4.

Effect of LPS and FnIII‐1c activation on the expression of genes involved in adipogenic differentiation of hMSCs. MSCs were cultured under adipogenic medium with and without 1 µg/mL LPS or FnIII‐1c for 7, 14 and 21 d for all the experiments mentioned below. The mRNA levels of (A) lipoprotein lipase, (B) Fatty acid‐binding protein 4 (FABP4), and (C) peroxisome proliferator‐activated receptor gamma (PPAR‐γ) were evaluated using real‐time PCR. (D) Characterization of hMSCs was conducted using Oil Red O staining (scale bar = 100 mm). Data are presented as mean ± SEM of ratios relative to β‐actin levels (n = 3). *P < .05, **P < .01, ***P < .001, ****P < .0001