FIGURE 7.

Effect of TLR4 knockdown on the expression of genes involved in osteogenic, adipogenic and chondrogenic differentiation. MSCs were transfected with psiRNA‐hTLR4 (SiTLR4 group) and psiRNA‐LucGL3 (control group). The mRNA levels of (A) Runt‐related transcription factor 2, (B) osteocalcin, (C) bone morphogenetic protein‐2, (D) lipoprotein lipase, (E) Fatty acid‐binding protein 4 (FABP4), (F) peroxisome proliferator‐activated receptor gamma (PPAR‐γ), (G) SOX9, (H) type II collagen (COL2A1) and (I) Aggrecan (ACAN) were evaluated using real‐time PCR for (A‐C) osteogenic (D‐F) adipogenic and (G‐I) chondrogenic differentiation, respectively. (J) ALP activity of treated groups undergoing osteogenic differentiation was evaluated, MSCs were cultured in DMEM medium in the control group (Con). Data are presented as mean ± SEM of ratios relative to β‐actin levels (n = 3). *P < .05, **P < .01, ***P < .001, ****P < .0001