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. 2021 Jun 4;7:133. doi: 10.1038/s41420-021-00521-0

Fig. 5. NR4A1 enhances the transactivation of the MKP7 promoter and physically associates with its promoters at two putative sites.

Fig. 5

A Sketch for NR4A1 putative binding site in MKP7 promoter. B A diagram of MKP7 promoters with different lengths was designed for luciferase reporter construction. C The relative luciferase activity of MKP7 promoters with different lengths exhibited in both OV and NC cells. D and E ChIP analysis were exploited to detect the physical association between NR4A1 and the promoter region of MKP7; the exogenous NR4A1-HA expression with adenoviral infection in MIN6 cells was associated with chromatin at some specific DNA sequences, after chromatin immunoprecipitation with anti-HA antibodies, the pulled-down DNA fragments were subjected to PCR analysis with specific pairs of primers. The two putative NR4A1 binding sites (−55 to −50, −1637 to −1632) in the MKP7 promoter regulatory sequence were confirmed with specific PCR amplification. These data represented the means of three independent experiments, **P < 0.01, ***P < 0.001 vs. ns. Error bars were shown as SD values.