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. 2021 Jun 4;12(6):576. doi: 10.1038/s41419-021-03803-8

Fig. 2. CRC-secreted exosomal miR-21-5p is transferred to endothelial cells and induces angiogenesis and vascular permeability in vitro.

Fig. 2

A Transmission electron microscopy of exosomes derived from SW480 and SW620. Scale bars represent 200 nm. B Expression of GM130 and TSG101 in cell lysates or exosomes extracted from SW480/mock, SW480/miR-21-5p, SW620/NC, or SW620/zip-miR-21-5p by Western blot. Expression levels were normalized to β-actin. C Expression of miR-21-5p and pri-miR-21 in HUVECs incubated with exosomes derived from SW480/mock, SW480/miR-21-5p, SW620/NC, or SW620/zip-miR-21-5p by qRT-PCR. Mean ± SEM are provided (n = 3). D Effect of SW480/mock exosomes, SW480/miR-21-5p exosomes, SW620/NC exosomes, and SW620/zip-miR-21-5p exosomes on the proliferation of HUVECs. Mean ± SEM are provided (n = 3). E Effect of SW480/mock exosomes, SW480/miR-21-5p exosomes, SW620/NC exosomes, and SW620/zip-miR-21-5p exosomes on the migration of HUVECs by Boyden chamber. Scale bars represent 50 µm. Mean ± SEM are provided (n = 3). F Effect of SW480/mock exosomes, SW480/miR-21-5p exosomes, SW620/NC exosomes, and SW620/zip-miR-21-5p exosomes on tube formation ability of HUVECs. Scale bars represent 50 µm. Mean ± SEM are provided (n = 3). G Effect of SW480/mock exosomes, SW480/miR-21-5p exosomes, SW620/NC exosomes, and SW620/zip-miR-21-5p exosomes on the permeability of the HUVEC monolayers to rhodamine-dextran by in vitro permeability assay. Mean ± SEM are provided (n = 3). H The number of GFP + CRC cells invaded through the HUVEC monolayers pre-treated with SW480/mock exosomes, SW480/miR-21-5p exosomes, SW620/NC exosomes, and SW620/zip-miR-21-5p exosomes. Scale bars represent 50 µm. Mean ± SEM are provided (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. NS represents no significant difference.