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. 2021 Jun 4;11:11837. doi: 10.1038/s41598-021-91235-x

Table 1.

Characteristics of the specimens that were discordant among the Mindray CL-900i anti-SARS-CoV-2 IgG, BioMérieux VidasIII anti-SARS-CoV-2 IgG, and Roche Elecsys Anti SARS-CoV-2 antibody testing.

Specimen number% Record of PCR positive test prior to study£ Date of blood and naso-pharyngeal swab specimens’ collection at time of study PCR test at time of study Mindray CL-900i anti-SARS-CoV-2 IgG* BioMérieux VidasIII anti-SARS-CoV-2 IgG** Roche Elecsys anti SARS-CoV-2*** Symptomatic at time of study# Severity at or right after time of study$
Test result Test result Ct value Optical density Test result Optical density Test result Optical density Test result
20 N/A 10-Aug-20 Negative 3.76 Negative 5.86 Positive 0.48 Negative No N/A
21 N/A 10-Aug-20 Negative 111.72 Positive 0.45 Negative 67.27 Positive No N/A
22 N/A 6-Aug-20 Positive 21.62 3.74 Negative 0.10 Negative 8.40 Positive Symptomatic Severe
29 N/A 27-Jul-20 Negative 5.81 Negative 1.08 Positive 5.62 Positive No N/A
37 N/A 10-Aug-20 Negative 7.77 Negative 0.47 Negative 3.66 Positive No N/A
40 N/A 29-Jul-20 Negative 20.24 Positive 0.01 Negative 0.16 Negative No N/A
50 N/A 11-Aug-20 Negative 31.25 Positive 0.04 Negative 0.09 Negative No N/A
61 N/A 29-Jul-20 Negative 135.05 Positive 0.45 Negative 38.01 Positive No N/A
71 N/A 28-Jul-20 Negative 4.17 Negative 0.88 Negative 3.01 Positive No N/A
82 N/A 10-Aug-20 Negative 71.76 Positive 0.02 Negative 0.08 Negative No N/A
126 N/A 10-Aug-20 Positive 26.95 10.50 Positive 0.15 Negative 0.18 Negative No N/A
128 N/A 10-Aug-20 Positive 33.81 220.63 Positive 0.62 Negative 7.72 Positive No N/A
129 N/A 10-Aug-20 Negative 16.60 Positive 0.07 Negative 0.08 Negative No N/A
131 N/A 13-Aug-20 Positive 28.32 36.22 Positive 0.46 Negative 9.23 Positive No N/A
137 N/A 10-Aug-20 Negative 4.72 Negative 0.46 Negative 10.98 Positive No N/A
147 N/A 10-Aug-20 Negative 39.57 Positive 0.94 Negative 10.92 Positive No N/A
174 N/A 29-Jul-20 Positive 24.09 12.05 Positive 1.29 Positive 0.12 Negative No N/A
182 N/A 29-Jul-20 Negative 11.22 Positive 0.35 Negative 0.07 Negative Not reported N/A
191 N/A 10-Aug-20 Negative 7.87 Negative 1.75 Positive 8.41 Positive No N/A
213 N/A 29-Jul-20 Negative 140.62 Positive 0.00 Negative 0.11 Negative No N/A
223 N/A 10-Aug-20 Negative 7.29 Negative 4.73 Positive 7.35 Positive No N/A
228 N/A 10-Aug-20 Negative 0.17 Negative 5.56 Positive 40.35 Positive No N/A
232 N/A 29-Jul-20 Positive 23.89 16.48 Positive 0.19 Negative 0.09 Negative No N/A
246 N/A 10-Aug-20 Negative 5.60 Negative 1.37 Positive 6.98 Positive No N/A
255 N/A 27-Jul-20 Negative 8.06 Negative 1.09 Positive 10.18 Positive No N/A
280 N/A 29-Jul-20 Positive 32.34 20.61 Positive 2.30 Positive 0.51 Negative No N/A
296 N/A 27-Jul-20 Negative 15.7 Positive 0.04 Negative 0.15 Negative No N/A
319 N/A 29-Jul-20 Negative 4.07 Negative 2.48 Positive 1.16 Positive No N/A
328 N/A 29-Jul-20 Negative 6.41 Negative 0.68 Negative 2.59 Positive No N/A
337 N/A 29-Jul-20 Negative 80.22 Positive 0.86 Negative 5.62 Positive No N/A
355 N/A 29-Jul-20 Positive 29.18 2.36 Negative 2.11 Positive 0.10 Negative No N/A
357 N/A 29-Jul-20 Negative 10.49 Positive 3.34 Positive 0.73 Negative No N/A
360 N/A 10-Aug-20 Negative 13.57 Positive 0.06 Negative 0.09 Negative No N/A
376 N/A 10-Aug-20 Negative 45.70 Positive 0.07 Negative 0.09 Negative No N/A
387 N/A 11-Aug-20 Positive 24.24 310.78 Positive 0.46 Negative 4.56 Positive No N/A

Ct cycle threshold, N/A not applicable, PCR polymerase chain reaction.

*Mindray CL-900i anti-SARS-CoV-2 IgG assay positive: optical-density cutoff index ≥ 10.0 vs. negative: cutoff index < 10.06.

**BioMérieux VidasIII assay positive: optical-density cutoff index ≥ 1.0 vs. negative: cutoff index < 1.07.

***Roche Elecsys Anti SARS-CoV-2 assay positive: optical-density cutoff index ≥ 1.0 vs. negative: cutoff index < 1.05.

%Specimen number consistent with Supplementary Table S1 in Supplementary Information.

£Date of first positive swab and Ct value are not available as there was no record for a prior positive swab for these specimens.

#Symptoms as reported in the medical record for this infection.

$Severity per WHO classification19. If N/A, no severity classification was conducted due to absence of serious symtoms to require hospitalization and severity assessment.