Table 1.
Treatment | Temperature (°C) | Incubation time (h) | Virus titer after treatment (PFU/ml) |
---|---|---|---|
– | Stock − 80 °C | – | 1–2 × 106 |
– | 4 °C | Up to one week | 1–2 × 106 |
– | 20 °C | 0.5 and up to 48 h | 0.8–1 × 106 |
– | 58 °C | 0.5 | No plaques |
No FA | 20 | 0.5 | 1.4 × 106 |
0.1% FA | 4 | 4 h | 200 |
0.1% FA | 20 | 0.5 | 20 |
0.5% FA | 20 | 0.5 | No plaques |
1% FA | 20 | 0.5 | No plaques |
2% FA | 20 | 0.5 | No plaques |
3.6% FA | 20 | 0.5 | No plaques |
Virus titer was determined by plaque assays (see Supplementary Figure S3).