Figure 2.

Off-target analysis of PE2 variants

(A and B) Six on-target sites and their 10 off-target sites were examined to have mutations with nuclease (spCas9, spCas9-SpG, spCas9-SpRY) (A) and PEs (wild-type PE2, PE2-SpG, and PE2-SpRY) (B) in HEK293T cells (detailed information is listed in Table S3). The specific ratio, which means the relative mutation frequency of on-target sites compared to that of off-target sites, is listed in Table S4. The numerical values of the mutation frequencies are described in Table S1, and information for pegRNAs is described in Table S2. Mean ± SEM of n = 3 independent biological replicates.