Figure 3.

Prime editing in HEK293T cells by PE3 and PE3b systems

(A–C) Comparison of prime editing and indel activities at the HEK2-2+1Gdel site (A), at the HEK4-4+1C>G site (B), and at the CUL3-3+1C>G site (C). The PAM sequences are shown in parentheses. The numerical values of the mutation frequencies are described in Table S1, and the information for pegRNAs is described in Table S2. Mean ± SEM of n = 3 independent biological replicates.