FIGURE 5.

Knockdown USP53 significantly promotes migration and invasion of ccRCC. (A, B) Cell wound healing ability was promoted at indicated time points after USP53 depleted compare with control through wound healing assay. In 786‐O cells (A) Caki‐1 cells (B). Scale bar 300 μm. Relative cell migratory distance of Caki‐1 USP53 overexpression and Control were quantified and statistically analyzed (right panel). (C, D) Transwell migration and invasion assays were performed in Transwell assays with or without Matrigel, and USP53 knockdown significantly inhibits the cell migration and invasion. (C) Representative Images of Transwell migration assay of USP53 depleted in 786‐O cells and Control cells for 10 h. Scale bar, 200 μm. (D) Representative images of Transwell invasion assay of USP53 depleted in Caki‐1 cells and Control cells for 24 h. Scale bar, 200 μm