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Establishment of the method for evaluating the inhibitory activity on ACE2-RBD interaction. (A) Schematic diagram of the principle of biotinylated binding assay. (B) Validation of ACE2-RBD interaction by SPR. The 1:1 binding model was used to assess binding kinetics. (C) RBD was coated on a 96-well plate. (D) ACE2 was coated on a 96-well plate. (E) Soluble recombinant ACE2 blocked the interaction between immobilized ACE2 and RBD. Data are shown as mean ± SEM of six independent assays.
