FIGURE 2.

Anticoagulant activities of monovalent aptamers HD1 and M08, their mixture with HD22, and their bivalent aptamers with different length of linker. A, The normalized clotting time of fibrinogen after mixing with thrombin in the presence of HD1, M08, or its mixture with HD22. B, The normalized clotting time of fibrinogen after mixing with thrombin in the presence of bivalent aptamers comprising linked M08 and HD22 with different‐length linkers. A reaction of thrombin and fibrinogen was used as an internal standard and its clotting time was defined as 1. Final concentrations: aptamer = 2.5 nM, thrombin = 2.5 nM, and fibrinogen = 0.4 mg/mL