FIGURE 3.

Neutralizing ability of antidotes against M08‐T15‐HD22. A, Sequences of M08‐T15‐HD22 and all tested antidotes. B, The normalized clotting time of fibrinogen after mixing thrombin and M08‐T15‐HD22 in the presence of complementary single‐stranded DNA as the antidote. A reaction of thrombin, M08‐T15‐HD22, and fibrinogen was used as an internal standard and its clotting time was defined as 1. Final concentrations: aptamer = 5 nM, thrombin = 2.5 nM, fibrinogen = 0.4 mg/mL, and antidote = 5 nM