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. 2021 Jun 5;10(6):e1287. doi: 10.1002/cti2.1287

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© 2021 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Immunohistochemical detection of pulmonary ILC and Th cells in COPD patients and controls. T‐BET/GATA3/RORγt in brown, CD3 in green and antibodies against CD20, CD56, CD68 and mast cell tryptase (exclusion cocktail) in black. Staining was performed in COPD GOLD II patients and smoking and never‐smoking controls. T‐BET⁺CD3⁻ILC1 (in brown, blue arrow) and T‐BET⁺CD3⁺Th1 cells (in brown with green border, red arrow) negative for exclusion markers (a). GATA3⁺CD3⁻ILC2 (blue arrow) and GATA3⁺CD3⁺Th2 cells (red arrow) negative for exclusion markers (b). RORγt⁺CD3⁻ILC3 (blue arrow) and RORγt ⁺CD3⁺Th17 cells (red arrow) negative for exclusion markers (c). Scale bar: 20 µm.