Figure 2.

Panx 1 blockers reduced ATP release induced by hypotonic challenge in Schwann cells.

(A, B) Time course of ATP and LDH release from cultures treated in the Iso, Hypo, and Triton conditions. The Triton group was used as a positive control in the LDH assay. (C, D) ATP and LDH release were assessed under different conditions. Schwann cells were pretreated with carbenoxolone (100 μM) and probenecid (100 μM) for 60 minutes before exposure to the indicated solutions for 12 minutes. Dimethyl sulfoxide at the same volume in isotonic solution was used as a Ctrl. Data are expressed as the mean ± SEM (n = 4). *P < 0.05, **P < 0.01 (one-way analysis of variance or Student’s t-test). CBX: Carbenoxolone; Ctrl: control; Hypo: hypotonic; Iso: isotonic; LDH: lactate dehydrogenase; Vehicle: dimethyl sulfoxide.