Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 16;16(5):899–904. doi: 10.4103/1673-5374.290911

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2021 Neural Regeneration Research

This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.

PMC Copyright notice

siRNA knockdown of Panx 1 attenuates hypotonic solution-induced ATP release.

(A) mRNA levels of Panx 1 in Schwann cells treated with scrambled siRNA (NC) and Panx 1-siRNA (1–3). (B, C) Panx 1 protein bands in RSC96 cells in the scrambled (control) and Panx 1-siRNA (1–3) groups. (D) The relative expression of Panx 1 in isotonic solution after transfection with scrambled siRNA (Ctrl) or in hypotonic (Hypo) solution after transfection with scrambled and siRNA-Panx 1 (#3) is shown. (E) ATP release was assessed using scrambled siRNA and Panx 1-siRNA-treated Schwann cells. (F) Lactate dehydrogenase (LDH) release was assessed under the same conditions as ATP release. Data are expressed as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01. #P < 0.05, vs. control group (one-way analysis of variance or Student’s t-test). Panx 1: Pannexin 1; siRNA: small interfering RNA.