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. 2020 Nov 16;16(5):916–923. doi: 10.4103/1673-5374.297090

Figure 3.

Figure 3

Effects of acupuncture treatment on the expression of GSK-3β in the dorsal raphe nucleus in the rat model of D-galactose-induced aging.

(A) The protein levels of total GSK-3β, GSK-3β-pTyr216 and GSK-3β-pSer9 in the dorsal raphe nucleus were detected by western blot assay. GAPDH was used as a loading control. (B–D) Quantitative results for GSK-3β (B), GSK-3β-pTyr216 (C) and GSK-3β-pSer9 (D) levels. The data are expressed as the mean ± SD (n = 6). **P < 0.01, vs. control group; ▲▲P < 0.01, vs. model group; #P < 0.05, ##P < 0.01, vs. PEA group (one-way analysis of variance followed by the least significant difference test). Control: Normal; Model: D-galactose-induced aging; PEA + inhibitor: D-galactose-induced aging + preventive electroacupuncture + 5-aza-2′-deoxycytidine (an inhibitor of DNA methyltransferase); Inhibitor: D-galactose-induced aging + 5-aza-2′-deoxycytidine; PMA: D-galactose-induced aging + preventive manual acupuncture; PEA: D-galactose-induced aging + preventive electroacupuncture. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GSK-3β: glycogen synthase kinase-3β.