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. 2020 Nov 16;16(5):916–923. doi: 10.4103/1673-5374.297090

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Copyright: © 2021 Neural Regeneration Research

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Effects of acupuncture treatment on the expression of DNMT1 in the dorsal raphe nucleus in the rat model of D-galactose-induced aging.

The expression level of DNMT1 in the dorsal raphe nucleus (DRN) in the model group and inhibitor group were significantly decreased. PEA, PMA and PEA + inhibitor treatments all increased the expression levels of DNMT1, and PEA treatment was more effective than PMA or PEA + inhibitor treatment. (A) The immuoreactivity of DNMT1 in the DRN was detected using immunofluorescence staining. Arrows indicate DNMT1-positive cells. (B) The protein levels of DNMT1 in the DRN detected by western blot assay. GAPDH was used as a loading control. (C) Quantitation of DNMT1-positive cells. (D) Quantitation of protein expression of DNMT1. The data are expressed as the mean ± SD (n = 3 for immunofluorescence staining, n = 6 for western blot assay). *P < 0.05, **P < 0.01, vs. control group; ▲P < 0.05, ▲▲P < 0.01, vs. model group; #P < 0.05, ##P < 0.01, vs. PEA group (one-way analysis of variance followed by the least significant difference test). Control: Normal; Model: D-galactose-induced aging; PEA + inhibitor: D-galactose-induced aging + preventive electroacupuncture + 5-aza-2′-deoxycytidine; Inhibitor: D-galactose-induced aging + 5-aza-2′-deoxycytidine; PMA: D-galactose-induced aging + preventive manual acupuncture; PEA: D-galactose-induced aging + preventive electroacupuncture. GSK-3β: Glycogen synthase kinase-3β; DNMT1: DNA methyltransferase 1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.