Fig. 3.

Minimal residual lesions are well-vascularized and not hypoxic. a H&E (left) and IF staining for CD31 (right) on sections of a representative HER2/neu-Prim1 orthotopic MRL. b IF staining for CD31 on sections of an intact MTB;TetO-Wnt1 primary tumor (left) or MRL (right). c Fluorescence microscopy of liver (top) or orthotopic MRL (bottom) from same mouse for H2B-eGFP, i.v. injected Hoechst 33342 and Lectin-AF647, and immunofluorescence staining for pimonidazole. Arrowhead denotes positive pimonidazole staining surrounding the hepatic vein; arrow denotes absence of pimonidazole staining surrounding the hepatic artery. Scale bars: 100 μm for all images shown