Fig. 2. Proteome coverage for HeLa digest and single HeLa cells. (A) FAIMS method optimization using 0.5 ng aliquots of HeLa protein digest. Protein groups identified with different detection and fragmentation methods and using two or three FAIMS CVs. (B) Protein groups and unique peptides identified from single HeLa cells with and without FAIMS. Error bars indicate standard deviations based on 3 replicate measurements.
