Influence of buffer on the 125I-iodination and 211At-astatination of 4-chlorobenzeneboronic acid (1). RCYs determined by radio-HPLC analysis of the crude producta.
| Solvent (v : v) | RCYa (%) | |
|---|---|---|
| 125I | 211At | |
| 0.9% NaCl/DMF (85 : 15) | 38 ± 3 | 78 ± 13 |
| PBS/DMF (85 : 15) | 21 ± 2 | 31 ± 7 |
| Sodium acetate buffer 0.1 M pH 7.5/DMF (85 : 15) | 40 ± 4 | 84 ± 8 |
| Sodium citrate buffer 0.5 M pH 7.5/DMF (85 : 15) | 0b | — |
| Tris buffer 0.5 M pH 7.5/DMF (85 : 15) | 35 ± 8 | 47 ± 9 |
a
Standard conditions: (1) (0.25 mM), Cu(OTf)2pyr4 (10 mM), 1,10-phenanthroline (10 mM), [125I]NaI or [211At]NaAt (1–5 MBq), 30 min, 100 μL, 23 °C, n = 3.
b
Cu(OTf)2pyr4 (5 mM), 1,10-phenanthroline (5 mM).