Fig. 2. Live/dead bacterial viability test by confocal laser scanning microscope (CLSM) imaging. (A) Confocal images of APNB-treated bacteria and COS-7 cells post live/dead staining (scale bar = 20 μm). (B) Confocal images of ABP-treated bacteria and COS-7 cells post live/dead staining (scale bar = 20 μm). (C) Confocal images of NB-treated bacteria and COS-7 cells post live/dead staining (scale bar = 20 μm). (D) Confocal images of control group post live/dead staining (scale bar = 20 μm). The PACT treatment is conducted under 660 nm light irradiation (20 mW cm−2) for 15 min. [APNB] = [NB] = 0.5 μM. [Calcein-AM] = 5 μM. [PI] = 5 μM. λex = 490 nm for Calcein-AM, and λex = 530 nm for PI.
