Fig. 3. Inhibition of AspH by 2OG derivatives. (a) The AspH IC₅₀-values for the 2OG derivatives 17 (black diamonds), 29 (red squares), 33 (orange circles), and 34 (green triangles) depend on the 2OG concentration. AspH inhibition assays were performed in triplicate as described in the ESI† using 3, 200, 400, and 600 μM 2OG. IC₅₀-values are summarized in ESI Table S1;† (b) representative dose–response curves used to determine IC₅₀-values for the 2OG derivatives 17 (black diamonds), 29 (red squares), 33 (orange circles), and 34 (green triangles) at a 2OG assay concentration of 3 μM 2OG. Three dose–response curves each composed of technical duplicates were independently determined using SPE-MS AspH inhibition assays, performed as described in the ESI† and manifest high Z′-factors⁴⁰ and signal-to-noise ratios (ESI Fig. S3†).