Steady-state kinetic parameters for AspH. Maximum velocities (v^app_max), Michaelis constants (K^app_m), turnover numbers (k_cat), and specificity constants (k_cat/K_m) of His₆-AspH_315–758 for 2OG and the 2OG derivatives 16 and 41^a,b.

	AspH co-substrate	v app [μM s−1]	K app [μM]	k cat [s−1]	k cat/Km [μM−1 s−1]
1	2OG	15.0 × 10−3 ± 0.9 × 10−3	1.3 ± 0.3	0.17 ± 0.03	0.13 ± 0.03
2	3-Methyl-2OG (16)	6.9 × 10−3 ± 0.2 × 10−3	0.27 ± 0.04	0.08 ± 0.01	0.30 ± 0.06
3	4-Carboxyphenylglyoxylic acid (41)	4.0 × 10−3 ± 0.3 × 10−3	61.9 ± 11.0	0.04 ± 0.01	0.65 × 10−3 ± 0.2 × 10−3

^aMean of three independent runs (n = 3; mean ± SD).

^bAspH assays were performed as described in the ESI using 0.1 μM His₆-AspH_315–758 and 2.0 μM hFX-CP_101–119 (ESI Fig. S1d) as a substrate.