Fig. 5. (a) Scheme showing the cellular uptake of X-SNA probe and ratiometric detection of lactate in living cells. (b) Confocal images of X-SNA treated HeLa cells after incubation with lactate medium or inhibitor. Ratiometric images clearly showed colour change after treatments. (c) Showing mean fluorescence ratio of with X-SNA treated cells after treatment with either lactate medium or inhibitor, as determined by flow cytometry. (d) Confocal ratiometric images and (e) calibration curve of X-SNA treated L-02 cells in the presence of different external lactate concentrations.
