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Metal binding by PilB. Purified PilB was incubated with increasing concentrations of divalent ions (Ca2+, Mg2+, and Mn2+), and binding was quantified by ThermoFluor. (A) Metal binding by PilB. (B) Metal binding by PilBD319A with an inactive MIDAS. The results are the average ± SDs from three independent experiments.
