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. 2021 May 28;118(22):e2025759118. doi: 10.1073/pnas.2025759118

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Fig. 1. — CoPoP/PHAD liposomes convert His-tagged HA trimers into particles. Sequence identity of representative recombinant H3 HA trimers available from the IRR with HA from the heterologous H3N2 mouse or ferret challenge strains used in this study. HA from A/canine/Illinois/11613/2015, shown in red, was selected for mouse vaccination and A/HongKong/1/1968 challenge (A) while HA from A/Victoria/361/2011 was selected for ferret immunization and A/Texas/50/2012 challenge (B). (C) Binding of His-tagged H3 HA trimers from Illinois/2015 to CoPoP/PHAD or PoP/PHAD (no Cobalt) liposomes after incubation at RT for 1 h. Particle diameter (D) and polydispersity (E) of liposomes after incubation with Illinois/2015 HA trimer. (F) Cryo-TEM images of CoPoP/PHAD liposomes before antigen binding or following incubation with Illinois/2015 H3 HA trimers at a HA-to-CoPoP mass ratio of (G) 1:4 (select HA trimers indicated by black arrows) or (H) 2:1. (C–E) Data show mean ± SD from n = 3 samples.