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. 2021 May 26;118(22):e2019699118. doi: 10.1073/pnas.2019699118

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Fig. 1. — NICD/p53^−/− mutant organoids grow spheroid shaped and exhibit regenerative properties. (A) Brightfield and immunofluorescence for Ki67 of NICD/p53^−/− organoids (Upper) and control organoids (Lower) (Left, Scale bar, 250 µm.); three-dimensional reconstitution of confocal z-stack images (Middle) and optical section (Right) of immunofluorescence for Ki67 (red) and DAPI (blue). (Scale bar, 50 µm.) Surface buds in the controls are marked by dashed lines. The Inset shows dividing cells. (B) Violin plot of differentially regulated genes (DRGs) in NICD/p53^−/− compared to control organoids for cell-type-specific gene signatures. (C) Violin plot of stem cell genes differentially regulated in NICD/p53^−/− compared to control organoids. (D) Relative mRNA expression of stem cell markers. (E) Violin plot of fetal/regenerative markers differentially regulated in NICD/p53^−/− compared to control organoids. (F) Relative mRNA expression of fetal/regenerative genes. (G) Volcano plot of fetal/regenerative makers differentially regulated in NICD/p53^−/− organoids. (*P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001.)