Fig. 5.

In vivo depletion of T cells reverses the improvement of vessel perfusion and the inhibition of tumor growth induced by DLL1 overexpression in EO771 breast tumor model. EO771-R1 and EO771-L1 breast tumors were prepared as described in Fig. 1. Mice were randomly assigned to two groups and treated with anti-CD8 and anti-CD4 antibodies or control IgG (200 μg/mouse) on days 6 (the corresponding tumor sizes were 3 to 4 mm in diameter), 8, and 14 post–tumor cell inoculation, respectively. (A) The tumor growth curves and tumor weight. (B and C) Tumor blood vessel perfusion and vessel density in EO771-R1 and EO771-L1 breast tumors without or with T cell depletion. (Scale bars, 100 μm.) The significance was determined by one-way ANOVA. Data are from one experiment representative of two independent experiments with similar results (n = 7 to 8 mice per group). **P < 0.01, ***P < 0.001.