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. 2021 Apr 30;118(22):e2004838117. doi: 10.1073/pnas.2004838117

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Fig. 1. — Assessment of off-targets indels within gene-drive populations targeting AGAP007280. Targeted amplicon sequencing was conducted for 18 off-target sites predicted from the CIRCLE-seq for gRNA-7280. The frequency (in percent) of reads containing indels are displayed as a heatmap. WT served as the negative control. Three different gene-drive populations were assessed for off-target mutations over several generations (as indicated below the heatmap): vas-7280^CRISPRh, zpg-7280^CRISPRh, and zpg-7280^SDGD. For vas-7280^CRISPRh and zpg-7280^CRISPRh, each generation was comprised of two biological replicates (cages 1 and 2). For WT and zpg-7280^SDGD, each generation comprised one biological replicate only. For zpg-7280^SDGD this was due to population collapse of one of the biological replicates (cage 2) after eight generations (which was intended as part of the study).