Fig. 2.
Assessment of off-target indels within a gene-drive population targeting dsx. Amplicon sequencing was conducted for 133 in silico-predicted off-target sites for gRNA-dsx. The percentage of reads containing indels are displayed. A wild-type mosquito population served as control. Individuals from two generations of a zpg-dsxCRISPRh population experiment were assessed. For each generation two biological replicates were sequenced. Three further off-target sites were sequenced, but not displayed here due to high levels of genetic polymorphism at the cleavage site across both the control and experimental populations.