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Indel frequency at off-target 4 and off-target 6 for vas-7280CRISPRh. The top 10 indels witnessed in the G3 were individually followed through subsequent generations to assess if frequency changed over time. The frequencies of the indels were normalized to the total number of reads containing indels for that sample. There are 11 indels displayed, as the top 10 indels in CT1 (cage trial 1) were not identical to those witnessed for CT2 (cage trial 2).
