Fig. 3.

Mating with B2t¹ mutant males suppresses female fertility. (A) Schematic of the female fertility suppression assay performed in a 30 × 30 × 30 cm cage. B2t¹ males were allowed to mate with 15 wild-type (WT) females for different lengths of time (first cross). The males were then removed. In some experiments, 15 WT males were then added (second cross) and retained for the duration of the experiment. After 3 d, the females were allowed to blood feed. They were then kept in the cage for an additional 4 d before transferring individual females to vials so that progeny could be scored. (B) The percentage of females that produced progeny following the first and second crosses with the indicated numbers of WT or B2t¹ males. n ≥ 5. Means ± SEMs. Statistics were performed using one-way ANOVA with Tukey’s multiple comparisons tests. ***P < 0.001. (C) The percentage of females that produced progeny following the first cross with 15 B2t¹ males for different lengths of time and a second cross (of the same females) with 15 WT males. n ≥ 3. Means ± 95% CI. The red curve indicates the fitted curve.