Fig. 4.

B2t¹ mutant males compete with wild-type (WT) males to suppress female fertility. (A) Schematic of the mating competition assay performed in a 30 × 30 × 30 cm cage. B2t¹ and WT males were introduced simultaneously to mate with 15 WT females. The males were kept in the cages with females until egg collection. (B) The percentage of females that produced progeny following mating with males of indicated B2t¹ to WT ratios. n ≥ 5. Means ± SEMs. Statistics were performed using one-way ANOVA with Tukey’s multiple comparisons tests. ***P < 0.001. (C) Curve fitting for the same dataset from B. n ≥ 5. Means ± 95% CI. The dashed lines indicate the point at which the B2t¹ to WT ratio (∼5.6) leads to a 50% decrease in the number of fertile females (47%) from the 94% female fertility obtained when the females are exposed to WT males only.