Table 1.
Life table for B2t1, B2t1/+, and +/+ mosquitoes
| Developmental stage | Genotype | No. | Developmental time (days) |
| Eggs (from B2t1/+ ♀ × ♂) | 400 | − | |
| B2t1 | 85.7 ± 1.3 | − | |
| Fourth instar larvae | B2t1/+ | 164.0 ± 6.1 | − |
| +/+ | 93.7 ± 0.9 | − | |
| B2t1 | 80.3 ± 2.3 | 6.2 ± 0.2 | |
| Pupae | B2t1/+ | 155.7 ± 8.7 | 6.4 ± 0.2 |
| +/+ | 90.0 ± 1.5 | 6.5 ± 0.2 | |
| B2t1 | 78.7 ± 3.5 (♀ to ♂ ratio: 0.873 ± 0.024) | 8.4 ± 0.4 | |
| Adults | B2t1/+ | 155.3 ± 8.4 (♀ to ♂ ratio: 0.897 ± 0.045) | 8.5 ± 0.4 |
| +/+ | 91.7 ± 1.3 (♀ to ♂ ratio: 0.887 ± 0.067) | 8.5 ± 0.2 |
A total of 400 eggs from the cross of B2t1/+ heterozygous males and females were hatched for each group. Three groups were assayed. B2t1/+, B2t1, and +/+ progeny were distinguished and sorted using the eye fluorescent marker during the fourth instar larval stage. The number of pupae and adults, the genotypes, and the time of development are indicated. Means ± SEMs. There were no significant differences between the various genotypes in development times, adult female to male ratios, and in the number of Bt21 and Bt21/+ genotypes at any developmental stage. Statistics were performed using one-way ANOVA with the Tukey’s multiple comparisons test.