Fig. 1. (a) Emission spectra of Pro-DC (1 μM) in aq. HEPES buffer solution (10 mM, pH 7.4) upon irradiation with a blue LED lamp (λirr = 400 nm, 1.0 mW cm−2) at 25 °C. Irradiation times: 0, 1, 4, 6, 10, 20, 30, 40, 50, 60 and 70 minutes, respectively. The emission (λEm = 660 nm with λExt = 640 nm). (b) Relative change in emission intensity (F/F0) at λEm 600 nm as a function of time for Pro-DC on irradiation with a 400 nm LED and in the dark: the red coloured trace represents changes on irradiation with 400 nm light and the blue colored trace represents the situation when Pro-Dc was kept in the dark.
